Non-Small Cell Lung Carcinoma Tissue Identification of Genes Differentially Expressed in Normal Lung

Using a magnet-assisted subtraction technique, 17 complementary DNA (eDNA) clones were isOlatedthat were expressed in the normal lung but were decreased or lost In the corresponding tumor tissue of a non small cell lung carcinoma patlent@The luck of expression of six magnet assisted subtraction technique eDNA clones in three additional non-small cell lung cardnoma cases indicates their possible relevance for non-small cell lung carcinoma. Two eDNA clones revealed homology to genes ape cifically expressed in lung, @e., pulmonary surfactant-associated protein B and the receptor for advanced glycosylation end products of proteins. Three cDNA clones showed identity to eDNA sequences encoding cal modulin-like protein, glutamine synthetase, and cytoskeletal 13-actln.One cDNA clone is identical to a recently described human expressed sequence tag whose gene is still unknown.